Focus Group “DIO mouse model and diet composition”
The use of obese and diabetic mouse models for investigating causes and characteristics of diabetes and obesity and potential therapeutic strategies for these diseases is a central component of research projects within the ICEMED Alliance. Diet-induced obese (DIO) mice are widely used as a model organism for common obesity and insulin resistance. However, there is no general standard procedure for generating DIO mice and therefore, reported characteristics, dynamics and therapeutic responses are very diverse.
Factors such as
mouse strain (diet-sensitive or not)
diet (high-fat diet, high-fat/high-sucrose diet, vendor etc.)
onset of high-fat diet feeding (at weaning or later)
duration of high-fat diet feeding
gender of mice
number of mice in a cage
maintenance conditions (animal facility, temperature, hygienic status)
are recognized as very important influencing characteristics of generated DIO mice. Standardizing all these conditions will be impossible.
The Focus Group “DIO Mouse Model” aims to summarize current protocols for generating DIO mice and respective DIO mice characteristics as reported from partners within ICEMED and from laboratories selected from scientific publication platforms.
In a next step, the Focus Group will analyze this information and make general recommendations to the ICEMED Alliance for generating DIO mice for certain outcome expectations (e.g. body fat content, insulin resistance or glucose intolerance).
The Focus Group will also serve as a forum for ICEMED scientists to discuss and communicate their data and experiences with one another, and the group will provide support to the ICEMED scientists regarding protocols/operating procedures for High Fat Diet and diet-induced obese and diabetic mouse models.
The work of the Focus Group is a work in progress. The Partner Platform was recently established at the ICEMED website and provides an internal forum for ICEMED scientists and Focus Group members to exchange and deposit protocols or data for internal use.
Focus Group members are scientists from partners within the ICEMED Alliance with long-lasting experiences in generating and investigating DIO mouse models:
Dr. Mauricio Berriel Diaz, DKFZ Heidelberg
Dr. Johannes Beckers, HMGU
Dr. Carola Meyer, HMGU
Prof. Dr. Mathias Treier, MDC Berlin
Dr. Jan Rozman, German Mouse Clinic (advisory member)
Ingrid Fischer, HMGU
To contact the Focus Group:
Dr. Juliane Kampe, Ph.D.
ICEMED Alliance – Imaging and Curing Environmental Metabolic Diseases
PhD project of the Icemed Focus Group (Ingrid Fischer):
In the context of the Helmholtz Alliance ICEMED, a Focus Group on “DIO mouse models and diet composition” came to life. Ingrid’s role as a PhD-Student is to create a guideline that contains recommendations on how to optimally establish overweight in mice for research on diabetes and obesity.
For her PhD studies Ingrid will be collaborating with different ICEMED partners, collecting and evaluating data from previous mouse DIO research. Subsequently she will be setting up a multi-center based animal DIO experiment in different laboratories of the ICEMED partners around Germany. This project aims to identify novel players and confounders in the development of diet-induced obesity in mice.
Mathias Treier, Max-Delbrück-Centrum (MDC) Berlin
Johannes Beckers, Helmholtz Zentrum München (HMGU)
Carola Meyer, Helmholtz Zentrum München (HMGU)
Mauricio Berriel Diaz, German Cancer Research Center (DKFZ)
Juliane Kampe, Helmholtz Zentrum München (HMGU)
Ingrid Fischer, Helmholtz Zentrum München (HMGU)
ICEMED Focus Group “DIO mouse models”: